pspCas13b crRNA design tool

Beta version

Input your DNA Sequence:

Spacer Length:
5' Oligo Adapter:
3' Oligo Adapter:
Good Sequence:
Bad Sequence:
Filtered Motifs:
Advanced Options

If you are using this Cas13 crRNA design server in your research, please cite:
'Design principles of PspCas13b for potent and off-target-free RNA silencing' by Hu et al, 2023, (under review).

Copyright @ 2023 Peter MacCallum Cancer Centre, Australia.